Model-based kinetic parameters estimation in batch Pullulan fermentation using Jaggery as substrate
Paper in scientific journal
V. S. Rama Krishna Ganduri, Ushakiranmayi Mangamuri, M. Vijayalakshmi, Sudhakar Poda
Department of Biotechnology, K L University, Green Fields, Vaddeswaram, Guntur District, Andhra Pradesh; Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjunanagar, Guntur, Andhra Pradesh; Department of Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, Guntur, Andhra Pradesh, India
Jan. 1, 2016
Journal of Chemical and Pharmaceutical Research, 2016, 8(3):217-224
The research was focused on validation of unstructured, mathematical, kinetic model data obtained in batch shakeflask studies of Pullulan produced by Aureobasidium pullulans MTCC 2195. Logistic (L), Logistic Incorporated Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models were used for kinetic parameters estimation. Very good fit of the data was observed between the experimental data and modelled data in biomass growth, substrate consumption and product formation kinetics. Estimated kinetic parameters like μmax, X0, Xm, α, β, γ, and η, were compared for initial Jaggery and Sucrose (as substrate) concentrations of 50, 75 and 100 g/L. Furthermore, all these parameters were predicted accurately with reasonable R2 values.